PTM Viewer

For each protein all PTMs are highlighted by default in the respective protein sequence (right-hand side). One can adjust a selection of PTMs in the PTM table on the left-hand side. In addition, functional protein domains and sites can be underlined if desired.

In the PTM table per PTM the PTM position and type is indicated, as well as the plain peptide sequence that was identified by mass spectrometry. The respective proteomics study is indicated by a number, providing a link to consult the experimental details. Additional PTM meta-data includes various confidence measures such as peptide score provided by search algorithms, posterior error probability (PEP), precursor mass deviation (in ppm) and modification site probability. The available confidence meta-data can be consulted in the extended PTM table by clicking SHOW CONFIDENCE. However, in the default PTM table, a color-coding of confidence is provided with green indicating high confidence, olive medium confidence, grey low confidence, and no color an unassigned confidence. More details regarding this confidence assignment can be consulted in the tutorial or the Plant PTM Viewer manuscript.

Besides confidence measures, log2 fold changes between two conditions with significance values (P- or Q-values) are shown if provided in the respective publication. Log2 fold changes are colored in heatmap-like gradient (green = induced, red = repressed) and significant values are highlighted in green. To determine significance, we employed the threshold used in the respective publication. For more details on the quantitative measurements we refer to the experimental details and respective publication, as methodologies can differ.

On the bottom of the page one can send the whole protein or a part of the protein (i.e. a functional domain) to PTM Blast. This will display aligned protein sequences that potentially report aligned PTMs.

PTMs

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Results

PTM Type	Mod AA	Pos	Peptide	Exp ID	Log2 FC	P/Q val	Score Type	Ident Score	Loc Prob	PEP	Mass Error (ppm)
ph	S	197	SEMHTTLQQGGRSPK	100			XCorr	2.883	0.999	5.845e-2
				109					1.000
				114			MaxQuant score	197.850	1.000	3.990e-35
ph	S	203	SMNSPTTSQPK	114			MaxQuant score	164.680	0.998	4.259e-11
ph	S	207	SMNSPTTSQPK	88			MASCOT Score	34.000	0.938		-0.060
ph	S	207	SMNSPTTSQPK	114			MaxQuant score	102.870	0.843	1.740e-13
ph	S	218	DGIQNSNSFLSQGK	88			MASCOT Score	39.000	0.994		-0.100
ph	S	218	DGIQNSNSFLSQGK	114			MaxQuant score	60.698	0.936	3.358e-3
ph	S	221	DGIQNSNSFLSQGK	114			MaxQuant score	115.040	0.992	5.489e-5
ub	K	239	MDHGSESVKR	40			MaxQuant Score	62.463	1.000	2.000e-3	0.545
ph	S	251	VDDSGSGPLRTESGLK	83			MASCOT Score	37.000			0.000
ph	S	251	VDDSGSGPLRTESGLK	100			XCorr	2.664	0.893	1.077e-2
ph	S	258	TESGLKSEILK	114			MaxQuant score	190.370	1.000	0.000e+0
ph	S	345	VGDGGSPKDSDR	88			MASCOT Score	35.000	0.993		-2.750
ph	S	345	VGDGGSPK	88			MASCOT Score	42.000	1.000		-1.660
ub	K	397	SHKNSEIGKKAR	40			MaxQuant Score	45.280	0.833	4.000e-3	-0.110
ph	S	419	SGSNQGVSWPGR	114			MaxQuant score	126.780	0.805	3.684e-5
ph	S	424	SGSNQGVSWPGR	114			MaxQuant score	68.171	1.000	5.673e-3
ph	S	472	CVATSPGSTR	88			MASCOT Score	31.000	0.991		-0.280
ph	S	472	CVATSPGSTR	114			MaxQuant score	177.440	1.000	8.905e-9
ph	S	475	CVATSPGSTR	85			XCorr	2.219	0.843	1.465e-2
ph	S	475	CVATSPGSTR	88			MASCOT Score	31.000	0.991		-0.280
ph	S	478	SAPSPGSGGNVSK	88			MASCOT Score	64.000	1.000		-0.100
ph	S	481	SAPSPGSGGNVSK	88			MASCOT Score	69.000	1.000		-0.260
ph	S	481	SAPSPGSGGNVSK	114			MaxQuant score	68.168	0.991	3.507e-3
ph	S	484	SAPSPGSGGNVSK	88			MASCOT Score	69.000	1.000		-0.260
ph	S	489	SAPSPGSGGNVSKDGQQR	88			MASCOT Score	29.000	1.000		0.780
ub	K	490	SAPSPGSGGNVSKDGQQR	40			MaxQuant Score	40.798	1.000	1.000e-3	0.067
ph	S	503	NAGAGGVSEVLAAVKDEK	114			MaxQuant score	143.860	1.000	1.336e-8
ub	K	564	KSNNVFQGSSSSASPR	40			MaxQuant Score	110.560	1.000	2.084e-23	0.336
ph	S	573	SNNVFQGSSSSASPR	83			MASCOT Score	42.000			0.000
ph	S	574	SNNVFQGSSSSASPR	88			MASCOT Score	67.000	0.872		-0.230
ph	S	575	SNNVFQGSSSSASPR	88			MASCOT Score	53.000	0.995		-0.160
ph	S	575	SNNVFQGSSSSASPR	106			MASCOT Score	38.000
ph	S	577	KSNNVFQGSSSSASPR	88			MASCOT Score	77.000	0.981		-0.380
			KSNNVFQGSSSSASPR	114			MaxQuant score	239.930	1.000	0.000e+0
			SNNVFQGSSSSASPR	83			MASCOT Score	92.000			0.000
				85			XCorr	2.775	1.000	4.780e-7
				88			MASCOT Score	84.000	1.000		-0.720
				100			XCorr	5.462	0.999	1.064e-9
				106			MASCOT Score	58.000
				109					0.988
				111a	0.339	0.000		96.210			0.823
				111b	0.339	0.000		96.210			0.823
				111c	0.006	0.000		96.210			0.823
				111d	0.006	0.000		96.210			0.823
ph	S	602	ISQSSLTSEK	114			MaxQuant score	87.667	0.923	1.623e-3
ph	S	617	TLEVPLTEGSGNKLIVK	114			MaxQuant score	124.690	0.994	3.985e-10
ph	S	617	TLEVPLTEGSGNK	88			MASCOT Score	43.000	1.000		-0.740
ph	S	631	GRSPAQSVSGGSLEDPAPVNSR	44			XCorr	3.855
				83			MASCOT Score	51.000			0.002
				84a			XCorr	4.980	0.975	5.430e-8
				84b			XCorr	4.130	0.983	8.720e-5
				85			XCorr	4.023	1.000	9.451e-12
				88			MASCOT Score	90.000	1.000		-0.280
				100			XCorr	3.655	0.999	6.092e-4
				111a	0.375	0.000		62.440			0.550
				111b	0.375	0.000		62.440			0.550
				111c	0.339	0.000		62.440			0.550
				111d	0.339	0.000		62.440			0.550
				114			MaxQuant score	190.260	1.000	0.000e+0
ph	S	635	GRSPAQSVSGGSLEDPAPVNSR	83			MASCOT Score	26.000			-0.001
				88			MASCOT Score	52.000	1.000		1.060
				100			XCorr	3.655	0.861	6.092e-4
			SPAQSVSGGSLEDPAPVNSR	114			MaxQuant score	391.760	0.891	0.000e+0
ph	S	637	GRSPAQSVSGGSLEDPAPVNSR	84b			XCorr	4.130	0.797	8.720e-5
			GRSPAQSVSGGSLEDPAPVNSR	88			MASCOT Score	52.000	1.000		1.060
			SPAQSVSGGSLEDPAPVNSR	88			MASCOT Score	34.000	0.992		-0.240
			SPAQSVSGGSLEDPAPVNSR	114			MaxQuant score	404.430	1.000	0.000e+0
ph	S	640	GRSPAQSVSGGSLEDPAPVNSR	61a			MASCOT Score	25.000	1.000
			GRSPAQSVSGGSLEDPAPVNSR	83			MASCOT Score	25.000			-0.000
			SPAQSVSGGSLEDPAPVNSR	114			MaxQuant score	160.360	0.996	6.637e-29
ph	S	649	GRSPAQSVSGGSLEDPAPVNSR	88			MASCOT Score	49.000	1.000		0.630
			SPAQSVSGGSLEDPAPVNSR	88			MASCOT Score	46.000	1.000		0.400
				106			MASCOT Score	31.000
				109					0.999
				111a	0.867	0.000		62.960			1.625
				111b	0.867	0.000		62.960			1.625
				111c	1.459	0.000		62.960			1.625
				111d	1.459	0.000		62.960			1.625
				114			MaxQuant score	384.830	1.000	0.000e+0
ph	S	652	SPAQSVSGGSLEDPAPVNSRVSSPVHAVK	100			XCorr	5.738	0.872	1.271e-7
ph	S	653	SPAQSVSGGSLEDPAPVNSRVSSPVHAVK	100			XCorr	5.738	0.872	1.271e-7
			VSSPVHAVK	38			MASCOT Score	44.900			1.652
				60			MASCOT Score	40.390			0.438
				85			XCorr	2.567	0.998	3.370e-2
				88			MASCOT Score	35.000	0.994		-0.040
				100			XCorr	2.603	0.997	2.658e-4
				109					1.000
				111a	-2.016	0.000		31.290			-0.258
				111b	-2.016	0.000		31.290			-0.258
				111c	1.782	0.000		31.290			-0.258
				111d	1.782	0.000		31.290			-0.258
				114			MaxQuant score	172.260	1.000	5.046e-5
ph	S	699	DILTGSQEAAGSPLVAGDER	88			MASCOT Score	51.000	0.995		-0.890
ph	S	705	DILTGSQEAAGSPLVAGDER	83			MASCOT Score	98.000			0.000
				85			XCorr	3.131	1.000	1.444e-4
				88			MASCOT Score	66.000	1.000		0.520
				100			XCorr	5.593	1.000	3.796e-8
				106			MASCOT Score	58.000
				109					0.972
				111a	-0.083	0.000		90.820			1.284
				111b	-0.083	0.000		90.820			1.284
				111c	1.047	0.000		90.820			1.284
				111d	1.047	0.000		90.820			1.284
				114			MaxQuant score	335.360	1.000	0.000e+0
ph	S	731	GTSSLGNEFK	114			MaxQuant score	84.658	0.851	2.048e-3
ph	S	732	GTSSLGNEFK	114			MaxQuant score	110.540	0.992	2.156e-4
ph	S	789	SPVASPSVSQPPNSVMNENSTVGNNTK	100			XCorr	4.202	0.795	3.616e-4
ub	K	865	DKSSNSDTEDLQR	40			MaxQuant Score	59.227	1.000	3.787e-4	0.671
ph	S	866	DKSSNSDTEDLQR	114			MaxQuant score	176.010	1.000	8.515e-16
ph	S	867	SSNSDTEDLQR	106			MASCOT Score	22.000
ph	S	869	SSNSDTEDLQR	85			XCorr	3.191	0.997	1.483e-2
				88			MASCOT Score	66.000	0.945		-0.580
				100			XCorr	3.578	0.825	1.615e-3
				106			MASCOT Score	41.000
				109					0.999
				111a	0.279	0.000		43.280			-0.134
				111b	0.279	0.000		43.280			-0.134
				111c	-1.133	0.000		43.280			-0.134
				111d	-1.133	0.000		43.280			-0.134
				114			MaxQuant score	244.260	1.000	0.000e+0
ph	S	884	LVDQCLESNDNSDGVVASPALPTK	83			MASCOT Score	39.000			-0.001
				84a			XCorr	4.290	0.903	8.074e-3
				111a	0.931	0.000		22.020			0.700
				111b	0.931	0.000		22.020			0.700
				111c	0.919	0.000		22.020			0.700
				111d	0.919	0.000		22.020			0.700
ph	S	888	LVDQCLESNDNSDGVVASPALPTK	83			MASCOT Score	38.000			0.002
				84a			XCorr	4.000	0.916	2.887e-2
				84b			XCorr	5.420	0.992	2.619e-4
				85			XCorr	2.997	0.999	6.549e-3
				111a	0.588	0.000		27.790			0.908
				111b	0.588	0.000		27.790			0.908
				111c	0.589	0.000		27.790			0.908
				111d	0.589	0.000		27.790			0.908
ph	S	894	LVDQCLESNDNSDGVVASPALPTK	83			MASCOT Score	39.000			-0.001
				84a			XCorr	4.000	0.966	2.887e-2
				84b			XCorr	5.420	0.999	2.619e-4
				85			XCorr	4.307	0.999	3.128e-11
				100			XCorr	3.422	1.000	5.259e-4
				106			MASCOT Score	43.000
				111a	0.914	0.000		31.020			1.249
				111b	0.914	0.000		31.020			1.249
				111c	-1.879	0.000		31.020			1.249
				111d	-1.879	0.000		31.020			1.249
				136	-0.203	0.708	MASCOT score	130.800			0.085
ph	T	899	LVDQCLESNDNSDGVVASPALPTK	111a	0.588	0.000		27.790			0.908
				111b	0.588	0.000		27.790			0.908
				111c	0.589	0.000		27.790			0.908
				111d	0.589	0.000		27.790			0.908
ph	S	910	ILNDSDSGELKDIK	85			XCorr	4.356	1.000	4.167e-10
				88			MASCOT Score	29.000	1.000		-1.140
				109					1.000
				111a	1.223	0.000		53.400			0.853
				111b	1.223	0.000		53.400			0.853
				111c	1.279	0.000		53.400			0.853
				111d	1.279	0.000		53.400			0.853
				114			MaxQuant score	187.560	1.000	2.073e-23
			ILNDSDSGELK	59			MASCOT Score	24.000			-0.473
				83			MASCOT Score	33.000			-0.001
				88			MASCOT Score	46.000	1.000		-0.400
				106			MASCOT Score	26.000
ph	S	912	ILNDSDSGELKDIKTDVK	38			MASCOT Score	45.920			-0.490
			ILNDSDSGELKDIK	18a			MaxQuant Score	91.355	0.986	2.720e-10	-0.647
				85			XCorr	4.356	1.000	4.167e-10
				88			MASCOT Score	35.000	1.000		-0.230
				114			MaxQuant score	230.290	1.000	0.000e+0
				136	0.148	0.606	MASCOT score	84.080			11.104
			ILNDSDSGELK	59			MASCOT Score	59.000			-0.709
				83			MASCOT Score	62.000			-0.002
				88			MASCOT Score	59.000	1.000		-0.330
				100			XCorr	3.775	0.996	1.411e-3
				106			MASCOT Score	25.000
				111a	0.195	0.000		46.720			0.190
				111b	0.195	0.000		46.720			0.190
				111c	-0.295	0.000		46.720			0.190
				111d	-0.295	0.000		46.720			0.190
ph	S	924	TDVKSEADCTSDSTKR	114			MaxQuant score	93.729	1.000	1.390e-5
ph	S	978	EEKPPTALSSELVK	100			XCorr	2.979	0.982	1.076e-3
ph	S	992	KVEEDVPVSSGISR	114			MaxQuant score	167.190	0.988	1.134e-15
ph	S	993	KVEEDVPVSSGISR	88			MASCOT Score	42.000	0.909		-0.610
ph	S	993	KVEEDVPVSSGISR	114			MaxQuant score	167.190	0.823	1.134e-15
ph	S	996	KVEEDVPVSSGISR	88			MASCOT Score	29.000	0.995		-0.220
ph	S	996	KVEEDVPVSSGISR	114			MaxQuant score	131.180	0.947	2.637e-5
ph	T	1034	IKQDCDTSVGAIK	88			MASCOT Score	35.000	0.946		-2.450
ph	S	1035	IKQDCDTSVGAIK	44			XCorr	3.216
ph	S	1035	QDCDTSVGAIK	114			MaxQuant score	197.290	0.934	1.974e-35
ph	S	1043	DTSAGLDSSVTK	59			MASCOT Score	46.000			-1.112
ph	S	1043	DTSAGLDSSVTK	114			MaxQuant score	171.260	1.000	2.918e-35
ph	S	1049	DTSAGLDSSVTK	114			MaxQuant score	194.300	0.860	2.046e-24
ph	S	1065	GKVEPVEGNLENSEVKER	88			MASCOT Score	58.000	1.000		-1.000
			GKVEPVEGNLENSEVK	100			XCorr	4.312	1.000	4.700e-5
			VEPVEGNLENSEVKER	109					1.000
			VEPVEGNLENSEVKER	114			MaxQuant score	163.450	1.000	5.556e-22
			VEPVEGNLENSEVK	100			XCorr	4.003	1.000	1.239e-4
ph	S	1081	ATPGLSPK	88			MASCOT Score	38.000	1.000		-1.650
				109					1.000
				114			MaxQuant score	82.008	1.000	8.563e-3
ph	S	1097	EAEDLERPNGPKTSDADGDEAGECTSAAR	85			XCorr	2.931	0.938	2.144e-8
				111a	0.088	0.000		62.850			0.619
				111b	0.088	0.000		62.850			0.619
				111c	1.023	0.000		62.850			0.619
				111d	1.023	0.000		62.850			0.619
			TSDADGDEAGECTSAAR	88			MASCOT Score	90.000	0.998		-0.470
			TSDADGDEAGECTSAAR	114			MaxQuant score	162.260	0.766	3.218e-32
ox	C	1107	TSDADGDEAGECTSAAR	47	-0.279	0.312
ph	T	1108	TSDADGDEAGECTSAAR	114			MaxQuant score	203.060	0.860	0.000e+0
ph	S	1109	TSDADGDEAGECTSAAR	85			XCorr	4.633	0.997	2.960e-3
				88			MASCOT Score	86.000	0.996		-0.160
				106			MASCOT Score	72.000
				114			MaxQuant score	326.380	0.971	0.000e+0
ph	S	1116	DASSVSAAASAGSEMDAR	88			MASCOT Score	29.000	0.984		0.040
ph	S	1118	DASSVSAAASAGSEMDAR	88			MASCOT Score	59.000	1.000		-0.920
ph	S	1122	DASSVSAAASAGSEMDAR	38			MASCOT Score	49.100
				59			MASCOT Score	74.000			-0.454
				88			MASCOT Score	73.000	1.000		-0.490
				114			MaxQuant score	131.180	1.000	6.120e-15
ph	S	1125	DASSVSAAASAGSEMDAR	88			MASCOT Score	73.000	1.000		-0.490
ph	S	1125	DASSVSAAASAGSEMDAR	114			MaxQuant score	180.030	1.000	0.000e+0
ph	S	1270	VLEDLASQR	114			MaxQuant score	74.944	1.000	6.538e-3
ph	S	1286	SGNPTNCTSDITNSFDQVR	114			MaxQuant score	207.430	0.999	0.000e+0
ub	K	1338	LDSSFQQVKLPSTGGR	40			MaxQuant Score	50.178	1.000	1.148e-5	-0.301
ph	S	1380	SGLPSQPSLSGIR	114			MaxQuant score	142.670	0.945	1.660e-6
ph	S	1382	SGLPSQPSLSGIR	114			MaxQuant score	129.040	0.884	6.568e-5
me1	R	1427	GDQPFPMIATRGPQR	123			MaxQuant Score	68.044	1.000	3.073e-6	0.375
ph	S	1573	QLSSSASLPLKEDQAR	114			MaxQuant score	99.392	0.927	1.319e-5
ph	S	1577	QLSSSASLPLKEDQAR	114			MaxQuant score	77.660	0.963	9.438e-5
ub	K	1581	QLSSSASLPLKEDQAR	40			MaxQuant Score	58.980	1.000	9.606e-8	0.699

Sequence

Length: 1613

MHGRVCERRHKSRRRHMLISSSRVIATVEGGGSSCLSLSSSTSFSKDGRKISVGDCALFKPPQDCPPFIGIIRLIIAEEEDKLKLGVNWLYRPTELKLGKGILLEAEPNELFYSFHEDNIPAASLLHPCKVAFLPRGVELPSGISSFVCRRVYDVTNERLWWLTDQDYIDDRQLEVDKLLCKTRSEMHTTLQQGGRSPKSMNSPTTSQPKDGIQNSNSFLSQGKGRKRERMDHGSESVKRERSSRVDDSGSGPLRTESGLKSEILKFTEKGGLVDSEGVEKLVQLMLPERNEKKIDLVGRAILAGVVAATDKFDCLSRFVQLRGLPVFDEWLQEVHKGKVGDGGSPKDSDRLVDDFLLVLLRALDKLPVNLNALQTCNIGKSVNHLRSHKNSEIGKKARSLVDTWKKRVEAEMDAKSGSNQGVSWPGRLSHGGRHSGGSAEANKTSSSHLHASKSVSVKQQVENNLKCVATSPGSTRSAPSPGSGGNVSKDGQQRNAGAGGVSEVLAAVKDEKSSSSSQSHNNSQSCSSEHAKTGNLCGKEDARSSTAGSTLKKCSGGSSRHRKSNNVFQGSSSSASPRGAGLSRSFSSHRNVPSEKISQSSLTSEKTLEVPLTEGSGNKLIVKLPNRGRSPAQSVSGGSLEDPAPVNSRVSSPVHAVKQELCDNNGREKNHSYRPNVSSVLNAESWQSNELKDILTGSQEAAGSPLVAGDERGGDLKDSDKASGNVKGTSSLGNEFKSGERHGGTLSSMNALIESCVRYSETNASLAGSDDVGMNLLASVAADEMSKSPVASPSVSQPPNSVMNENSTVGNNTKLMASDGLPHEQHQAVCTSVSTEQGEQHVSSSGTQLESEIKNESKTGDRDKSSNSDTEDLQRLVDQCLESNDNSDGVVASPALPTKAVKEKILNDSDSGELKDIKTDVKSEADCTSDSTKRVASSMLTECRDVSKKVDSVAVEQTPLEGVDDDKKEEKPPTALSSELVKKVEEDVPVSSGISRDMDAVSIGRPITEMVNNVAFNHMDQKDVKKIKQDCDTSVGAIKDTSAGLDSSVTKGKVEPVEGNLENSEVKERYSGLRATPGLSPKEAEDLERPNGPKTSDADGDEAGECTSAARDASSVSAAASAGSEMDARVEFDLNEGFDGDDAKHGDSNNFSGSVFLTPTPLQPVKTLPFPVAPVSSGTRASITVAAAAKGPFVPPEDLLRNKGAVGWRGSAATSAFRPAEPRKPQDVLLSINNTSTSDASTSAGKQTRTFLDFDLNVPDERVLEDLASQRSGNPTNCTSDITNSFDQVRSGVMGSALDHSSGGLDLDLNKVDDSTDMISYTMNSSHRLDSSFQQVKLPSTGGRRDFDLNDGPVGDDAAVEPSMVLNQHSRSGLPSQPSLSGIRVNGENMASFSTWFPAANAYSAVSMPPIMPERGDQPFPMIATRGPQRMLGPTTGVSSFTPEGYRGPVLSSSPAMPFQSTTFQYPVFPFGNSFPVTSANFPGASTAHMDSSSSGRACFPGVNSQILGPGVPVPSNYPRPYIVGLPNGGSNGGVLDNGAKWFRSGLDLNSGPGGHETEGRDESTLVARQLSSSASLPLKEDQARMYQMSGGVLKRKEPEGGWDGYRQSSWQ

ID	PTM Type	Color
ph	Phosphorylation	X
ub	Ubiquitination	X
ox	Reversible Cysteine Oxidation	X
me1	Monomethylation	X
	Multiple types	X

Domains & Sites

Clear highlighted range

**Interpro Domains**
IPR ID	Description	From	To
IPR001025	Bromo adjacent homology (BAH) domain	49	164
IPR003617	Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type	332	411
IPR017923	Transcription factor IIS, N-terminal	326	412

AT3G48050.1

Arabidopsis thaliana [ath]

protein SUO

77 PTM sites : 4 PTM types

PLAZA: AT3G48050

Gene Family: HOM05D002487

Other Names: 'shuttle' in chinese

Uniprot

F4JCS8

Link out to other resources with this protein ID : TAIR | PeptideAtlas | ARAPORT | PhosPhAt

PTMs

Sequence

Length: 1613

Domains & Sites

BLAST

Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)
A downloadable tutorial can be found here

AT3G48050.1

Arabidopsis thaliana [ath]

protein SUO

77 PTM sites : 4 PTM types

PLAZA: AT3G48050

Gene Family: HOM05D002487

Other Names: 'shuttle' in chinese

Uniprot

F4JCS8

Link out to other resources with this protein ID : TAIR | PeptideAtlas | ARAPORT | PhosPhAt

PTMs

Sequence

Length: 1613

Domains & Sites

BLAST

Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)A downloadable tutorial can be found here

Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)
A downloadable tutorial can be found here